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. 2007 Apr 3;8:92. doi: 10.1186/1471-2164-8-92

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Copyright © 2007 Li et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SAMD9 regulates cell proliferation. A. RNAi for SAMD9 is effective in reducing protein expression. A 200 kDa band for the SAMD9 fusion protein was detected in Cos-1 cells after co-transfection of pLP-EGFP-SAMD9 with either pSUPER-RNAi-SAMD9-mut9 or pSUPER-RNAi-EGFP, but it is not detected after co-transfection with either pSUPER-RNAi-SAMD9 or pSUPER-RNAi-SAMD9-2^nd. The expression of the EGFP-SAMD9 fusion protein was reduced in Cos-1 cells with RNA interference of SAMD9. B. Semi-quantitative RT-PCR for SAMD9 expression in MRC-5 cells. SAMD9 expression was reduced after RNA interference. C. Proliferation rate (Brdu incorporation percent) increased after RNA interference of SAMD9 in MRC-5 cells.