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. 2007 Apr 12;104(17):7277–7282. doi: 10.1073/pnas.0609259104

Fig. 2.

Fig. 2.

HRT promoter assay and HRT transcript levels, 18:1 content, TCV resistance, and viral replication in leaves containing normal or higher oleate levels. (A) Transient GUS assay. Leaves were infiltrated with untransformed cells (-ve) or Agrobacterium transformed with HRT–GUS fusion construct. The sid2 plants were treated with water (−) or SA (+) for 2 days before infiltration. Leaf discs (sid2) or whole leaves (ssi2 sid2) were processed for GUS histochemical staining as described before (45). (B) RT-PCR analyses showing basal-level expression of HRT in HRT ssi2 act1 and HRT ssi2 gly1 plants. The level of β-tubulin was used as an internal control to normalize the amount of cDNA template. The 18:1 levels are a mean of six independent replicates. (C) Systemic spread of TCV to uninoculated tissue (TCV-U) in TCV-inoculated plants. RNA was extracted from the uninoculated tissues at 18 days after inoculation and analyzed for the presence of the viral transcripts. Ethidium bromide staining of rRNA was used as a loading control. (D) Typical morphological phenotypes of mock- and TCV-inoculated HRT ssi2 act1 and HRT ssi2 gly1 plants. The susceptible plants showed crinkling, stunted bolt development, and drooping of bolts. Plants were photographed at 8 days after inoculation. (E) Effect of 18:1 infiltrations on viral replication in the inoculated leaf. Oleic acid (O, 1 mM) or water (W) was injected 24 h before or after (last two lanes) TCV inoculation, and the samples were harvested 72 h after inoculation. Ethidium bromide staining of rRNA was used as a loading control..