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. 2007 Apr 16;104(17):7033–7038. doi: 10.1073/pnas.0610627104

Fig. 2.

Fig. 2.

A purine at the center of the hairpin triloop (−11) is the major determinant of promoter recognition. (A) UV cross-linking of mini-vRNAP to 5-IdU-substituted and 32P end-labeled 20-mer P2–3 DNA. Above each lane, base and position substituted with 5-IdU are shown. Below each lane, Kd values obtained by SC using SPR for each singly 5-IdU-substituted DNA oligonucleotide are shown. For unsubstituted DNA, Kd = 2 nM. (B) Mini-vRNAP catalytic autolabeling with bGTP and [α-32P]ATP and unsubstituted or 5-IdU-substituted DNA at positions −12, −11, −10, −8, +1, +3. %A, percent activity relative to unsubstituted DNA. (C) GTP effect on mini-vRNAP UV-cross-linking to promoter-containing oligonucleotides substituted with 5-IdU at positions −12, −11, −10, +1, or + 3.