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Hairpin-sequence recognition by vRNAP. (A) Effect of promoter sequence on the affinity and salt resistance of vRNAP–promoter complexes. Binding affinity was determined by SC using SPR. (B) Role of H bond donors and acceptors at positions −11 and −8 on vRNAP–promoter interaction. Kd and salt resistance of the complex were determined as described. *, salt-resistant complex.
