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. 2007 Apr 16;104(17):7033–7038. doi: 10.1073/pnas.0610627104

Fig. 6.

Fig. 6.

Mini-vRNAP Trp-129 cross-links to −11 position of promoter DNA. (A) NTCB cleavage of C1–C5 RNAPs after UV cross-linking to −11 5-IdU- (Left) and −11 4-thio-T- (Right) substituted, 5′ 32P-labeled P2–3 DNA oligonucleotides. Stars indicate bands of interest. (B) NTCB cleavage of F120C, W129C, F145C, and G183C enzymes cross-linked to −11 5-IdU-labeled DNA. PhosphorImages of 10% SDS/PAGE are shown in A and B. (C) Effects of the W129A substitution on UV cross-linking to −11 5-IdU-labeled DNA (2), catalytic autolabeling (3), and binding affinity. 1, silver-stained gel; 2 and 3, PhosphorImages.