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. 2007 Apr 16;104(17):7033–7038. doi: 10.1073/pnas.0610627104

Fig. 7.

Fig. 7.

Mini-vRNAP promoter clearance. (A) P2–14, P2–15, and P2–16 DNA. Arrowheads show positions where the mini-vRNAP stalls transcription in the absence of CTP. (B) Mini-vRNAP was incubated with 5′ 32P end-labeled −11 IdU-substituted oligonucleotides P2–14, P2–15, or P2–16 in the presence of 1 μM GTP (lanes 1, 3, and 5), 1 μM each GTP, ATP, and UTP (lanes 2, 4, and 6), no NTPs (lanes 7 and 9), all four NTPs (lanes 8 and 10) followed by UV cross-linking. The relative cross-linking efficiency (%) and transcript length (RNAn) are shown below each lane. (C) Mini-vRNAP runoff transcription on a −16/−7 cross-linked stem (−DTT) or uncross-linked (+DTT) P2–32 template. Arrow, runoff product.