Fig. 3.

Analysis of viral protein production and infectious RV titers. (A) Viral protein production is not attenuated. NA cells were infected with rN2c or rΔLC8-N2c at an MOI of 5. At 24, 48, and 72 hpi, supernatants were harvested and RV particles were purified by centrifugation through 20% sucrose (see Materials and Methods). Viral structural proteins were visually quantified by Coomassie blue staining. (B) Viral attenuation in neuronal cell line supernatants harvested from NA cells from above were titered on NA (Upper) and BSR (Lower) in parallel.