Fig. 3.

Specific inhibition of protein synthesis by antisense HPS-ODNs. Quadruplicate bacterial cultures were grown for 6 weeks in 2 ml of 7H9 medium in the presence of single or multiple HPS-ODNs, as indicated, at concentrations of 10 μM for each HPS-ODN. The HPS-ODNs were added once at the start of the growth period. After 4 weeks, the 30/32-kDa complex proteins were analyzed in culture filtrates by immunoblotting and were quantitated densitometrically. Data below the immunoblot are the percentages of 30/32-kDa proteins present in each culture treated with HPS-ODN(s), compared with the amounts in an untreated culture. HP1, hairpin 1; HP2, hairpin 2.