Fig. 5.

HPS-ODN inhibition of M. tuberculosis multiplication in human macrophages. THP-1 cells seeded in 24-well tissue culture plates at a concentration of ≈5 × 10⁵ cells per well were infected with M. tuberculosis Erdman at an MOI of 1 or 10 and incubated for 5 days. All HPS-ODNs were added immediately after the 90-min infection period at a concentration of 10 μM. Triplicate cell cultures were lysed 3 h, 2 days, or 5 days after the infection for enumeration of cfu of M. tuberculosis. The maximal standard deviation for each of the data points at 5 days was 0.007 logs. Differences in cfu at 5 days between the group treated with three HPS-ODNs and either the untreated group or the group treated with mismatched HPS-ODNs were statistically significant at MOIs of both 1 and 10 (P < 0.0001 by ANOVA for all comparisons). In a second experiment that was similar to this one except that it lacked the group treated with mismatched HPS-ODNs and the cultures were assayed in duplicate, differences in cfu at 5 days between the group treated with HPS-ODNs and the untreated group were similar in magnitude to this experiment and statistically significant (P = 0.02 for MOI of 1 and P = 0.002 for MOI of 10). HP1, hairpin 1; HP2, hairpin 2.