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Immunoblot analysis of β2-AR expressed in COS-1 cells. (A) S120A and low-expressing β2-AR mutants (≈5 μg of solubilized membrane protein was loaded). (B) Wild-type β2-AR, V114A, L115A, and the compensatory mutants (≈2.5 μg of solubilized membrane protein was loaded). The arrows indicate the position of the fully glycosylated receptor. Mobility of molecular mass standards (kDa) is indicated next to the gel.
