Table 2.

Summary of ligand binding properties of single and compensatory mutant receptors

Receptor	Transmembrane helix	Kd, nM	95% confidence intervals	Bmax, pmol/mg
Wild type		2.1	1.70–2.40	18 ± 2.2
V114A	III	19	17.0–21.1	39 ± 1.6
S165V/V114A	IV/III	9.0	8.64–9.38	17 ± 0.6
S165V	IV	1.2	0.68–1.71	6.7 ± 0.4
L115A	III	2.4	2.06–2.89	24 ± 1.2
S161V/L115A*	IV/III	4.8	0.81–8.90	4.5 ± 0.7
S161V*	IV	2.0	0.80–3.14	2.7 ± 0.4

The values are expressed as the mean ± SE (n = 3–5 experiments).

*High nonspecific binding (15–20% of total binding).