Fig. 1.

Cytosolic J proteins of S. cerevisiae. Molecules per cell are as reported by Ghaemmaghami et al. (11). No data available is indicated by −. Phenotypes tested are as follows: cold sensitivity (C^S), resistance to diphtheria toxin (DT^R), very compromised for growth (very slow gr.), sensitive to cations (cation^S), and defective in uncoating CCVs (CCV def.). Information was obtained from the Saccharomyces Genome database, www.yeastgenome.org. J protein classes were taken from Walsh et al. (7). In the domain structure, arrows indicate the J domain fragments used (Apj1_1–161, Caj1_1–139, Cwc23_1–139, Djp1_1–127, Hlj1_1–179, Jjj1_1–128, Jjj2_1–132, Jjj3_1–124, Sis1_1–167, Swa2_362–668, Xdj1_1–146, Ydj1_1–134, and Zuo1_1–234). Numbers indicate amino acids in each full-length J protein. Regions in which these amino acids are highly represented include QS, G, GF, and GF/GM (listed by their standard single-letter amino acid code). J, J domain; Zn, zinc finger; CBD, clathrin-binding domain; UBA, ubiquitin association; TPR, tetratricopeptide repeat; Z, zuotin-like; CH, charged; TM, transmembrane.