Fig. 1.

Validation of Gluc secretion assay, linearity with cell number and time. Control (C2, circles) and DYT1 (D3, squares) fibroblasts were infected with a lentivirus vector encoding Gluc-IRES-cerulean to achieve infection of >90% cells. (A) Infected cells were plated 48 h after infection at different cell numbers per well, and luciferase activity in the medium was quantitated 24 h after plating. (B) Infected cells were plated at a density of 2.5 × 10³ cells per well, and luciferase activity in the media was quantitated 24, 48, and 72 h after plating. Mean values are shown ± SD; comparison of control and DYT1 secretion at 72 h; ∗, P < 0.001.