Fig. 5.

ER distribution of Gluc-YFP and PDI in control and DYT1 cells. Control (C1) and DYT1 (D1) cells were infected with Gluc-YFP lentivirus vector, and, 24 h later, cells were treated on ice with digitonin. Dual immunocytochemistry was carried out for Gluc-YFP (B and F) and the ER marker PDI (C and G) with merged images shown in the rightmost panels (D and H). Nuclei were stained with DAPI (A and E). (Magnification ×100.) (Scale bars, 10 μm.)