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Coimmune precipitation of torsinA and Gluc-YFP. Control (C4) (Left) and DYT1 (D2) (Right) cells were infected with lentivirus vector encoding Gluc-YFP-IRES-cerulean or YFP-IRES-cerulean (control), and, 72 h later, lysates were prepared and immune precipitated (IP) for torsinA. Lysates and torsinA-IP pellets were resolved by SDS/PAGE, and Western blotting was carried out with antibodies to GFP.
