Fig. 3.

The effect of free radical inactivation, the blockade of NO production and anti-oxidants on glutamate-induced ONOO⁻ formation by b.End3 cells. Cells were exposed to 10 mM glutamate for 24 h in the presence of increasing concentrations of: (A) UA, (B) l-NMMA and (C) N-AC. ONOO⁻ production was measured by the oxidation of dihydrorhodamine (DHR) to produce the fluorescent rhodamine. Results are presented as % increase in DHR oxidation compared to untreated cells. ^*p < 0.05 and ^**p < 0.01 compared to control group by one-way ANOVA with post hoc Dunnet's test.