FIG. 3.
Variation of ΔΨm of L. donovani promastigotes, as monitored by rhodamine 123 accumulation. The geometric mean from the fluorescence histograms was plotted against the HePC concentration. Parasites preincubated for 14 h with HePC were loaded with 0.3 μg/ml rhodamine 123, and the fluorescence level was measured by cytofluorometry. (Inset) Fluorescence histograms for the respective experiments. The drug concentration is shown at the side of each trace. Fully depolarized parasites were obtained by incubation with 10 mM KCN.