TABLE 1.
Comparison of IC50 and IC90 values for three reference clones of P. falciparum determined by the SYBR green I method and HRPII capture ELISA after 72 h of incubationa
| Drug and strain | IC50 (nM)
|
IC90 (nM)
|
||||||
|---|---|---|---|---|---|---|---|---|
| SYBR green I assayb
|
HRPII ELISAc
|
SYBR green I assay
|
HRPII ELISA
|
|||||
| Mean | 95% CId | Mean | 95% CI | Mean | 95% CI | Mean | 95% CI | |
| Chloroquine | ||||||||
| 3D7 | 8.1 | 6.6-10.9 | 7.5 | 4.9-8.6 | 26.4 | 12.5-55.6 | 33.2 | 16.8-55.6 |
| D6 | 6.1 | 4.7-7.9 | 5.9 | 4.5-7.2 | 16.1 | 12.4-20.9 | 15.8 | 16.5-19.7 |
| W2 | 35.1 | 26.2-46.9 | 33.2 | 21.2-47.2 | 125 | 61.9-252.7 | 128.7 | 104.5-162 |
| Mefloquine | ||||||||
| 3D7 | 9.4 | 6.2-14.2 | 8.4 | 5.8-13.9 | 29.8 | 23-38.6 | 25.6 | 14.2-37.6 |
| D6 | 6.4 | 3.2-12.8 | 4.7 | 3.8-5.7 | 23.3 | 14.7-36.8 | 18.7 | 14.9-32.3 |
| W2 | 3.1 | 0.7-13.2 | 1.9 | 1.3-2.8 | 12.2 | 4.4-33.8 | 8.0 | 4.3-10.6 |
| Quinine | ||||||||
| 3D7 | 6.9 | 4.5-10.5 | 5.9 | 5.5-6.2 | 63.7 | 20.5-195.5 | 42.6 | 23-0.62 |
| D6 | 6.6 | 3.8-11.3 | 6.7 | 4.8-9.2 | 12.4 | 6.7-22.8 | 13.2 | 7.1-23.5 |
| W2 | 22.2 | 7.4-66.7 | 15.1 | 4.5-24 | 116 | 98.2-136.7 | 128 | 93.5-182.9 |
a
None of the differences was significant by the Mann-Whitney U test.
b
Values are averages of n = 12.
c
Values are averages of n = 12.
d
CI, confidence interval.