TABLE 3.

Cost comparison of the SYBR green I-based and HRPII ELISA methods used to determine inhibitory concentrations

Method	Cost ($)/96-well platea					Time (h) per assay
	Antibodiesb	ELISA reagentsd	SYBR green Ie	SYBR green lysis bufferf	Total
SYBR green I	NAc	NA	0.20	0.68	0.88	1
HRPII ELISA	2.55	7.33	NA	NA	9.88	3

^aThe final costs are estimates based on the amounts of the reagents required to perform assays with one full 96-well plate and are calculated from the actual cost of ordering the reagent from a U.S.-based manufacturer. Shipping costs were not taken into consideration.

^bThe price includes those for both the IgM capture and the IgG detection antibodies.

^cNA, not applicable.

^dThe price is for blocking reagents, wash buffer reagents, and tetramethylbenzidine.

^eCost of the required amount of SYBG green I used per assay plate.

^fReagents required to make lysis buffer used in the SYBR green method.