TABLE 3.
Effects of IFN-γ and IFN-γ formulations on resistance to Brucella abortus in micea
| Formulation | CFU log10/spleen at the following time after infection:
|
Spleen wt (mg) | |
|---|---|---|---|
| 1 wk | 2 wk | ||
| Control | 5.2 ± 0.1 | 5.0 ± 0.2 | 125.0 ± 15.0 |
| NP | 5.1 ± 0.1 | 5.0 ± 0.1 | 175.0 ± 12.0b |
| IFN-γ | 5.0 ± 0.1 | 4.9 ± 0.0 | 158.0 ± 22.0b |
| IFN-γ-NPA | 4.4 ± 0.1b,c | 4.3 ± 0.1b,c | 231.0 ± 28.0b,c |
| IFN-γ-NPB | 5.1 ± 0.1 | 5.1 ± 0.1 | 216.0 ± 19.0b,c |
a
Mice received free IFN-γ or IFN-γ formulations at 104 IU/ml 24 h before infection as well as 2 and 4 days after infection. The numbers of B. abortus CFU per spleen were measured 1 and 2 weeks after infection. The spleen weights refer to the effects in control (noninfected) mice after 1 week following treatment with the IFN-γ formulations. Data are expressed as the means ± standard deviations of three experiments with three mice per group (1 week after infection; n = 9) or two mice per group (2 weeks after infection; n = 6).
b
P < 0.05 relative to the results for the control.
c
P < 0.05 relative to the results for mice treated with IFN-γ.