TABLE 1.
Primers used in this study
| Primer | Sequence (5′→3′) | Location | Reference or source |
|---|---|---|---|
| 5′ Conserved segment | GGCATCCAAGCAGCAAG | 5′CS integron 1 | 18 |
| intI 1L | ACATGTGATGGCGACGCACGA | intI1 | 32 |
| intI 1R | ATTTCTGTCCTGGCTGGCGA | intI1 | 32 |
| intI 2L | CACGGATATGCGACAAAAAGGT | intI2 | 32 |
| intI 2R | GTAGCAAACGAGTGACGAAATG | intI2 | 32 |
| intI 3L | GCCTCCGGCAGCGACTTTCAG | intI3 | 32 |
| intI 3R | ACGGATCTGCCAAACCTGACT | intI3 | 32 |
| OXA1F | CACAATACATATCAACTTCGC | blaOXA-1 | 29 |
| OXA1R | GTGTGTTTAGAATGGTGATCGC | blaOXA-1 | 29 |
| TEM-A2 | GTATCCGCTCATGAGACAATA | blaTEM-1 | 8 |
| TEM-EXT | TCTAAAGTATATATGAGTAAAC | blaTEM-1 | 8 |
| TnpM | GTCAGGGAAGACTCTATGACC | tnpM (Tn21) | This study |
| INT1FIN | CCTCACTAGTGAGAGGTAGGG | intI1 | This study |
| OXA1Fin | TATGGGAAAACTGGTGCAGGA | blaOXA-1 | This study |
| AAD2DI | CAGGAACCGGATCAAAGAG | aadA1 | This study |
| AADVD | AAGGTAGTCGGCAAATAATG | aadA1 | This study |
| INSB | CTTTGTCATGCAGCTCCACC | IS1 (insb) | This study |
| INSBR | GGTGGAGCATGACAAAG | IS1 (insb) | This study |
| ORFINV | CCAGACTGCTCCTGTATGACC | IS600 (ORFB) | This study |
| INT2INV | ACCTTTTTGTCGCATATCCGTG | intI2 | This study |
| DFRA1-FIN | CATCGAGCCGGAAGGTGATG | dfrA1 | This study |
| DFRA1R | GTTAGAGGCGAAGTCTTGGG | dfrA1 | This study |
| ORFXINV | CCATTTATGACGACCAATGC | ORFX | This study |
| AP 12h | CGGCCCCTGT | 44 | |
| ERIC1R | ATGTAAGCTCCTGGGGATTCAC | 42 | |
| ERIC2 | AAGTAAGTGACTGGGGTGAGCG | 42 |