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. 2007 Feb 26;51(5):1795–1803. doi: 10.1128/AAC.01447-06

TABLE 4.

Kinetic parameters for substrates of VV TK and human TK1 using new experimental compounds

Substrate	VV TK			Human TK1
Substrate	K_m (μM)^a	V_max (μM min⁻¹ mg⁻¹)^a	V_max/K_m^b	K_m (μM)^a	V_max (μM min⁻¹ mg⁻¹)^a	V_max/K_m^b
1	9.6 ± 5.5	120 ± 46	12	113 ± 20	130 ± 65	1.2
2	13 ± 5.2	119 ± 6	9	118 ± 14	98 ± 21	0.8
3	103 ± 2.9	91 ± 33.4	0.9	>200	<55	<0.28
4	11 ± 7.9	106 ± 7	9.6	>200	<55	<0.28
5	21 ± 1.1	91 ± 3	4.3	>200	<55	<0.28
6	97 ± 26	78 ± 7	0.8	>200	<55	<0.28
dThd	21 ± 12	302 ± 7	15	23 ± 13	334 ± 192	14.5
CDV	>200	<55	<0.28	>200	<55	<0.28

^a

Average of three or more determinations with standard deviations shown.

^b

Calculated efficiency using 0.5 μg of purified VV TK and TK1 in each assay.