Restriction analysis (PstI) (upper panel) and Southern blot hybridization with a blaTEM-52 probe (lower panel) of plasmid DNAs isolated from E. coli transconjugants. Lane 1, E. coli transconjugant 988SA01TC1; lane 2, E. coli transconjugant 2004/10101TC1; lane 3, E. coli transconjugant 2004/10256TC1; lane 4, E. coli transconjugant 05-00001TC1; lane 5, E. coli transconjugant 05-00590TC1; lane 6, E. coli transconjugant 05-00838TC1; lane 7, E. coli transconjugant 1043SA04TC1; lane 8, E. coli transconjugant 777SA01TC1; lane 9, E. coli transconjugant 260SA04TC1; lane 10, E. coli transconjugant 833SA04TC1; lane 11, E. coli transconjugant 153SA02TC1; lane 12, E. coli transconjugant 04-3486TC1; lane 13, E. coli transconjugant 977SA01TC1; lane 14, E. coli transconjugant pPAN-1 (the latter strain served as control and was previously published (14); lane M, markers (Smartladder; Eurogentec, Seraing, Belgium).