TABLE 2.
Primers and probes used for RT-PCR and real-time PCR
| Gene | Expt | Probe or primers | Sequence(s)a | Reference |
|---|---|---|---|---|
| EFB1 | RT-PCR | Primers | 5′-ATTGAACGAATTCTTGGCTGAC 5′-CATCTTCTTCAACAGCAGCTTG | 35 |
| ERG3 | RT-PCR | Primers | 5′-GGAAGAACCCATCAACTGGATGG 5′-GTGCCACTACTGCCATTCCA | 22 |
| ERG11 | RT-PCR | Primers | 5′-ATTGGTATTCTTATGGGTGGTCAACATAC 5′-CCCAATACATCTATGTCTACCACCACC | 16 |
| CDR1 | RT-PCR | Primers | 5′-TTTCTGGTGCCATGACTCCTGCTAC 5′-CAATATAAATGGCCAAAAAGAATACG | 25 |
| CDR2 | RT-PCR | Primers | 5′-GGGTATTGGCTGGTCCTAATGTGATTC 5′-CTAGCCAACCAGTAAAAGAAAATAGTAA | 25 |
| MDR1 | RT-PCR | Primers | 5′-AGAGCCATCACCGGTAACGACAG 5′-CCAACCAAAAATGAAAAGACCTGAAG | 25 |
| FLUI1 | RT-PCR | Primers | 5′-CACTGCCTTGGCTGGTAAC 5′-ACATCGTGCAAAAGGAAGAAC | 25 |
| CDR1 | Real-time | Probe | 6-FAM-TTAACCCATATGTCAGAAGTGCCCGGG-TAMRA | 6 |
| PCR | Primers | TTTAGCCAGAACTTTCACTCATGATT TATTTATTTCTTCATGTTCATATGGATTGA | 6 | |
| CDR2 | Real-time | Probe | 6-FAM-TCCCGGGTTTTGGATTTTCATGTACAGA-TAMRA | 6 |
| PCR | Primers | GGTATTGGCTGGTCCTAATGTGA GCTTGAATCAAATAAGTGAATGGATTAC | 6 | |
| MDR1 | Real-time | Probe | 6-FAM-TCGCAAGGCTAAAAGATTGAGAGCCATCA-TAMRA | 6 |
| PCR | Primers | TTACCTGAAACTTTTGGCAAAACA ACTTGTGATTCTGTCGTTACCG | 6 |
a
6-FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.