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DNA oligonucleotides for PCR gene amplification
| Enzyme and oligonucleotide | Direction | Sequence |
|---|---|---|
| MET2-cDNA | ||
| ML8890 | 5′ | GTCTCTAGAGCTAGCATGTCGGACAACACACCCACACCTCA |
| ML8891 | 3′ | CGCGGATCCAAGCTT TCATGATGTGTCCTCTGTTGG |
| MET2-gDNA fragment | ||
| ML9190 | 5′ | AAAACTGCAGGAATTCCATTCTTCTTCGCTCTACATCTCC |
| ML9191 | 3′ | CCGCTCGAGAAGCTTCTCCCTGGTAGCGAAGATAGC |
| Neomycin resistance cassette | ||
| ML8803 | 5′ | GGAAGATCTCTCGAGGCTGCGAGGATGTGAGCTGGA |
| ML8804 | 3′ | GGAAGATCTCTCGAGGGTTTATCTGTATTAACACGGAAGAGATG |
