FIG. 3.

DNA supercoiling activity of M. leprae DNA gyrase is sensitive to inhibition by gatifloxacin (GAT), sparfloxacin (SPX), and levofloxacin (LVX). Relaxed pBR322 (0.4 μg) was incubated with DNA gyrase reconstituted from GyrA (2 U, i.e., 600 ng) and GyrB (2 U, i.e., 700 ng) in the absence or in the presence of gatifloxacin, sparfloxacin, or levofloxacin. The reactions were stopped, and the DNA products were analyzed by electrophoresis in a 1% agarose gel. Lane a, relaxed pBR322 DNA; R and S, relaxed and supercoiled DNA, respectively.