TABLE 1.
Bacterial strains used in the present work and calculated MICs
| Bacterial species or strain | Relevant characteristic(s) (drug, MIC [μg/ml]) | Miltefosine susceptibility MIC (μM)a | Source or referenceb |
|---|---|---|---|
| S. pneumoniae | |||
| R6 | Unencapsulated laboratory strain; lytA+ | 5 | 33 |
| M32 | Unencapsulated laboratory strain; ΔlytA32 | 5 | 28 |
| 8249 | Serotype 19A, clinical isolate; multiresistant; lytA+ (penicillin, 6) | 6.25 | 24 |
| Spain23F-1 | Serotype 23F; lytA+ (penicillin, 1-2; tetracycline, 8; chloramphenicol, >8) | 6.25 | 30 |
| ATCC 6303 | Serotype 3; lytA+; Preceptrol strain | 6.25 | ATCC |
| S. pseudopneumoniae CCUG 49455T | Type strain; lytA+ | ND | 1; CCUG |
| Streptococcus sp. strain 782/96 | lytA+ (penicillin, 0.25; tetracycline, 128; erythromycin, >128) | ND | 32 |
| Streptococcus sp. strain 11923/96 | lytA+ (penicillin, 8; tetracycline, 0.5; erythromycin, 4) | ND | 32 |
| S. mitis NCTC 12161T | Type strain | 10 | NCTC |
| S. oralis NCTC 11427T | Type strain | 10 | NCTC |
| S. pyogenes CECT 985T | Type strain | 10 | CECT |
| S. agalactiae CECT 183T | Type strain | 20 | CECT |
| S. mutans CECT 479T | Type strain | 20 | CECT |
| S. sanguinis CECT 480T | Type strain | ND | CECT |
| S. uberis CECT 994T | Type strain | ND | CECT |
| E. faecalis | Vancomycin resistant (vanA) | 20 | F. Baquero, Hospital Ramón y Cajal, Madrid, Spain |
a
MICs are the means of three independent determinations; ND, not determined.
b
ATCC, American Type Culture Collection; CCUG, Culture Collection, University of Göteborg; CECT, Colección Española de Cultivos Tipo; NCTC, National Collection of Type Cultures.