FIG. 1.

Entry inhibition. Virus (hrR3 at a MOI of 0.02) was adsorbed to confluent Vero cells in 96-well plates for 1 h at 4°C. Free virus was rinsed off, and entry was initiated by shifting the temperature to 37°C. One hour later, remaining extracellular virus was inactivated, and six hours later, cells were lysed and infectivity was estimated by measuring the initial rates of β-galactosidase activity relative to the rate in mock-treated controls. Inhibitors were added just before the temperature shift and remained present throughout the entry period: •, TAT⁰; ○, TAT-Pd⁰; ▵, TAT-Cd⁰ in the presence of a 10× molar excess of DTT; □, DTT alone. The data points represent the means of triplicate determinations with standard errors of the means.