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Virus elution by heparin and bTAT-C0. Virus was adsorbed to fixed cells for 1 h at 37°C, and wells were rinsed three times over a period of 30 min at 37°C. Heparin (○) or bTAT-C0 (•) was added, and cultures were incubated for 1 h at 37°C, rinsed, and assayed for retained virus by ELISA. Data points represent means and standard errors of the means of triplicate determinations.
