FIG. 3.

EMSA with PmfR and DNA fragments. Assays were performed as described in Materials and Methods. (A) EMSA with 308-bp (12), 279-bp, and 228-bp 5′ DNA fragments derived with primer pairs shown in Table 1 from the 5′ regions of the purU, maO, and ssD genes, respectively. +, present; −, absent. (B) EMSA with the double-stranded oligonucleotide shown in panel C derived from the 5′ maO DNA region. (C) 5′ maO oligonucleotide. Underlined sequences indicate the PmfR binding site. (D) Position of the PmfR binding oligonucleotide with respect to the ribosome binding site and the GTG translational start codon of the maO gene. nt, nucleotides.