FIG. 2.

Generation of the fusion dioxygenase. The top line shows a representation of the bphA gene cluster of B. xenovorans LB400, encoding alpha and beta subunits (bphA1/bphA2), ferredoxin (bphA3), and ferredoxin reductase (bphA4). The hatched bphA1 segment between the restriction sites was exchanged with a PCR amplicon. The bottom line shows a representation of the alpha subunit. Catalytic and Rieske domains are shown in gray or are hatched, respectively. Horizontally connected vertical bars indicate sites encoding amino acid ligands of the Rieske iron-sulfur cluster ([2Fe-2S]) and of the active site mononuclear iron (mono-Fe). The part encoded by the amplicon and the flanking amino acid positions of the recipient subunit are indicated.