TABLE 3.
Substrate | BphA | Result for CBA
|
|||
---|---|---|---|---|---|
Retention time (min) | λmax (nm) | Identification | Concn (μM) | ||
2,2′-CB | B4h | 3.40 ± 0.01 | 204 ± 2 | 2-CBA | 44.8 ± 15.4 |
LB400 | 3.38 ± 0.01 | 204 ± 2 | 2-CBA | 121.0 ± 13.0 | |
3,3′-CB | B4h | 5.80 ± 0.05 | 231 ± 2 | 3-CBA | 25.4 ± 4.6 |
LB400 | 5.75 ± 0.04 | 231 ± 2 | 3-CBA | 9.9 ± 2.7 | |
4,4′-CB | B4h | NOa | NO | NO | NO |
LB400 | NO | NO | NO | NO | |
2,6-CB | B4h | 2.98 ± 0.05 | 206 ± 5 | 2,6-CBAb | 2.9 ± 1.4 |
LB400 | NO | NO | NO | NO | |
2,6,4′-CB | Bh4 | NO | NO | NO | NO |
LB400 | 5.58 ± 0.04 | 240 ± 2 | 4-CBA | 0.5 ± 0.3 | |
2,5,4′-CB | B4h | 5.84 ± 0.05 | 200 ± 5 | 2,5-CBAb | 0.5 ± 0.3 |
LB400 | NO | NO | NO | NO |
NO, not observed.
Identification based mainly on the retention time due to the uncharacteristic spectrum of the respective CBA.