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. 2007 Feb 23;73(8):2682–2689. doi: 10.1128/AEM.02523-06

TABLE 3.

CBAs formed from CBs by BphA-B4h or BphA-LB400 and BphBCD-LB400

Substrate BphA Result for CBA
Retention time (min) λmax (nm) Identification Concn (μM)
2,2′-CB B4h 3.40 ± 0.01 204 ± 2 2-CBA 44.8 ± 15.4
LB400 3.38 ± 0.01 204 ± 2 2-CBA 121.0 ± 13.0
3,3′-CB B4h 5.80 ± 0.05 231 ± 2 3-CBA 25.4 ± 4.6
LB400 5.75 ± 0.04 231 ± 2 3-CBA 9.9 ± 2.7
4,4′-CB B4h NOa NO NO NO
LB400 NO NO NO NO
2,6-CB B4h 2.98 ± 0.05 206 ± 5 2,6-CBAb 2.9 ± 1.4
LB400 NO NO NO NO
2,6,4′-CB Bh4 NO NO NO NO
LB400 5.58 ± 0.04 240 ± 2 4-CBA 0.5 ± 0.3
2,5,4′-CB B4h 5.84 ± 0.05 200 ± 5 2,5-CBAb 0.5 ± 0.3
LB400 NO NO NO NO
a

NO, not observed.

b

Identification based mainly on the retention time due to the uncharacteristic spectrum of the respective CBA.