FIG. 7.
Effect of using washed cultures on competitive exclusion of Desulfovibrio sp. strain Lac15 and Sulfurospirillum sp. strain KW. The following cultures were coinoculated at time zero: (A) washed Lac15 cells (2% [vol/vol]) and mid-log-phase KW (7% [vol/vol]); (B) mid-log-phase Lac15 (2% [vol/vol]) and washed KW cells (7% [vol/vol]); (C and D) washed Lac15 (2% [vol/vol]) and washed KW (7% [vol/vol]) cells. The concentrations of nitrate (□), nitrite (▴), lactate (♦), acetate (⋄), sulfide (•), and sulfate (○) and Eh (broken lines) are shown. All batch cultures were done in duplicate; average deviations are shown in panels A and B when error bars exceeded the size of the symbols. Panels C and D show duplicate cultures that received similar inocula but gave different results.