FIG. 1.

Construction of pMFY31-Pgap-xylA/xylB-Peno-tal/tktA and pMFY31-Pgap-xylA/xylB-Pgap-tal/tktA. Arrows indicate the transcriptional direction. The sparsely dotted arrow indicates the promoter of the Z. mobilis enolase gene (eno), while the densely dotted bar indicates the promoter of the Z. mobilis glycelaldehyde-3-phosphate dehydrogenase gene (gap). The blank bars indicate the E. coli TA (tal) and TK (tktA) genes, respectively. The dotted bars indicate the E. coli XI (xylA) and XK (xylB) genes, respectively. The solid line indicates vector plasmid pMFY31.