TABLE 1.
Expression of E. coli xylA, xylB, tal, and tktA in Z. palmae
| Plasmid or strain | Activitya of:
|
|||
|---|---|---|---|---|
| XIc (mU/mg) | XKd (U/mg) | TAe (U/mg) | TKf (U/mg) | |
| pMFY31 | <0.01 | <0.01 | 0.01 | 0.01 |
| pMFY31-Pgap-xylA/xylB-Peno-tal/tktA | 9.78 | 0.64 | 0.05 | 0.06 |
| pMFY31-Pgap-xylA/xylB-Pgap-tal/tktA | 9.33 | 0.81 | 0.25 | 0.26 |
| E. coli K-12b | 3.23 | 0.28 | 0.19 | 0.14 |
a
Cells were cultured in T medium containing 20 g/liter glucose and 100 μg/ml of ampicillin for 48 h at 30°C, and harvested cells were disrupted with an ultrasonic oscillator. The resulting supernatant was used as cell extract.
b
E. coli K-12 was cultured in Luria-Bertani medium for 24 h at 37°C with shaking, and cell extract was prepared from the harvested cells as described in footnote a.
c
One unit of XI produces 1 μmol of xylulose/min.
d
One unit of XK produces 1 μmol of NADH/min.
e
One unit of TA decreases 1 μmol of NADH/min.
f
One unit of TK decreases 1 μmol of NADH/min.