TABLE 1.
16S rRNA gene-targeted PCR screening of an Aroclor 1260-dechlorinating JN culture for known dechlorinating bacterial groups
| Dechlorinating group | PCR result at DNA dilution showna
|
Positive control | Primersb | |||||
|---|---|---|---|---|---|---|---|---|
| Direct
|
Nested
|
|||||||
| U | 1/10 | 1/100 | U | 1/5 | 1/50 | |||
| Dehalococcoides | + | + | + | D. ethenogenes 195 | DHC 1 (F) and DHC 1377 (R) (31) | |||
| Anaeromyxobacter | − | − | − | Anaeromyxobacter dehalogenans strain 2CP-C | A60-86F and A447-465R (21) | |||
| Desulfitobacterium | − | − | − | Desulfitobacterium sp. strain Viet 1 | Dd1 (F) and Dd2 (R) (23) | |||
| Desulfuromonas | − | − | − | Desulfuromonas michiganensis strain BB1 | BB1F and BB1R (41) | |||
| Dehalobacter | − | − | − | Dehalobacter sp. | deb 179f and deb 1007r (54) | |||
| Sulfurospirillum (formerly Dehalospirillum) | − | − | − | Sulfurospiriillum multivorans | JPDF 121 and JPDR 557 (50) | |||
| − | − | − | Sulfurospirillum halorespirans | DHSPM 576 and DHSPM 1210c | ||||
| Geobacter (SZ type) | − | − | − | Geobacter lovleyi strain SZ | 196F and 999R (58) | |||
| o-17/DF-1-type Chloroflexi | − | − | − | NDd | Clone of o-17 16S rRNA gene | 14F (22) and 1265R (61) | ||
a
U, undiluted.
b
References for some primers are shown in parentheses.
c
R. C. Ebersole and E. R. Hendrickson, U.S. patent application 20030077601.
d
ND, not determined. Nested PCR was not performed because the 14F primer shares the same recognition site as the bacterial universal primer 27F used in the initial round of PCR amplification.