TABLE 1.
Bacterial clades analyzed by qPCR, primers used, and amplicon sizes
| OTUa | Forward primer (5′→3′) | Reverse primer(5′→3′) | Amplicon size (bp) |
|---|---|---|---|
| 18 | TACAGCCTCCGAGCAACTG | ATCGACTCGCTCGCGATTC | 209 |
| 26 | GTGCTCAACCTCGAAGTGG | TTGGGCCACGGCAGCAGT | 163 |
| 37 | GAGGTGGTGCAGTTTGGC | GCGACCGATAGCCAACAAG | 162 |
| 52 | ATGTAGCCAAGGCGGGCG | GCCACTTCATCTGCAGTGC | 180 |
| 62 | GCAACTGAAGCTCGTCTCG | AACGGTACCTGCTGCTTCAT | 213 |
| 68a | CTAGCTGCCGTGCTAGCGA | ACCCGCTTTATTTGCGTCA | 126 |
| 68b | TTTTCCGAGCAGCACGGC | ACCAACTTCGATGCAGAGGT | 155 |
a
OTUs were based on a level of DNA sequence similarity of 97%.