TABLE 1.
Primers used for HXT3 cloning and integration in V5 hxt1-7Δ
| Primer | Sequence (5′ to 3′)a | Localization |
|---|---|---|
| HXT3P1 | GTGCGGGATccGAAGGCAATATC | −1128 |
| HXT3P2 | gatcggATCCATCATCACGTTCCTAGC | 2096 |
| I2HXT3 | aagtgacgggcgatgagtaagaaagaaataactgactcattagaCCATCATCACGTTCCTAGC | 2095 |
| C1HXT3ORF | GACACAGTGACATATGCACC | 168 |
| C2HXT3p | TTAAGCATGATCGTCTAGGC | −1689 |
| HXT3p426 | AacacaaaaacaaaaagtttttttaattttaatcaaaaaCTGAGTTAAACAATCATGAATTCAACTCC | −15 |
| HXT3t426 | GaatgtaagcgtgacataactaattacatgactcgagACGGTTTAGCGTGAAATTATTTCTTGCC | 1694 |
| C1HXT3ORF | GACACAGTGACATATGCACC | 168 |
| C′2HXT7p426 | gccaatacttcacaatgttcg | −125 |
a
Underlining indicates homology with the HXT7 terminator, uppercase letters indicate homology with HXT3, double underlining indicates homology with the p4H7 promoter, italics indicate homology with the p4H7 terminator, and boldface type indicates homology with the HXT7 promoter.