TABLE 1.
Bacterial strains and vectors used in this study
| Strain or vector | Genotype or relevant feature | Reference or source |
|---|---|---|
| 10403S | L. monocytogenes serotype 1/2a | 3 |
| CU1065 | B. subtilis W168 trpC2 attSPβ | J. D. Helmann |
| DH5α | E. coli supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab stock |
| pCR2.1-TOPO | Cloning vector from Invitrogen | |
| pDG780 | Contains a gram-positive kan resistance cassette | 12 |
| pKSV7 | Shuttle vector with gram-positive pE194ts ori | 22 |
| pLTV3 | Tn917 delivery vector | 4 |
| pMC1 | Gram-positive cat gene from pPL2 cloned into pCR2.1-TOPO | This study |
| pMC3 | Gram-positive cat gene from pMC1 cloned into pMMOrf | This study |
| pMC14 | Gram-positive kan gene from pDG780 cloned into pPL2 | This study |
| pMC25 | mariner delivery vector with L. monocytogenes promoter PP60 | This study |
| pMC30 | mariner delivery vector with L. monocytogenes promoter PactA | This study |
| pMC38 | mariner delivery vector with B. subtilis promoter PmrgA | This study |
| pMC39 | mariner delivery vector with B. subtilis promoter PkatA | This study |
| pMMOrf | Contains 5′ and 3′ ITR from Himar1 | 15 |
| pNF1100 | Contains a copy of Himar1 derived from pMEnt-neo | N. E. Freitag; 16 |
| pPL2 | Site-specific shuttle integration vector | 17 |
| pPL3e | pPL2 derivative with gram-positive ermC gene | 10 |