TABLE 3.
Comparison of transposon delivery vectors
| Delivery vector | Size of vector | Size of transposon (bp) | Total no. of mutants (CFU/ml ± SD)a | % ± SD of CFUs bearing vector |
|---|---|---|---|---|
| pLTV3(4) | 22.1 kb | 14,861 | (9.80 ± 2.00) × 104 | >50b |
| pMC38 | 8,172 bp | 1,395 | (1.73 ± 1.07) × 106 | 2.4 ± 1.7 |
| pMC39 | 8,291 bp | 1,395 | (1.08 ± 0.76) × 106 | 0.85 ± 0.01 |
a
The total number of mutants generated by each vector was obtained from the results of three independent libraries for pLTV3 and pMC38 and two independent libraries for pMC39. SD, standard deviation.
b
Estimated number.