TABLE 2.
Oligonucleotides used in this study
| Name | Sequence (5′→3′)a | Created restriction site | Application |
|---|---|---|---|
| AML005 | ATGGGCCCGTTTTGATCTCGTACAGTG | ApaI | Construction of pRV601 |
| AML006 | ATGATATCAATTCATCAGCAAGTTCTC | EcoRV | Construction of pRV601 |
| catKpnF2 | TGACTGGTACCGTTACCCTTATTATCAAGAT | KpnI | Amplification of cat-194 |
| catBspR2 | TGACTTTCGAATTGATCTGGAGCTGTAATAT | Amplification of cat-194 | |
| pJWinversFor | TAATACCTAGGCAGCTGGGCATGATGGAT | XmaJI | Amplification of p29TIVET |
| pJWinversRev | ACACCCTGCAGATGACTAGCTATCTATGATCAC | PstI | Amplification of p29TIVET |
| gusAFor | GAGAACTGCAGACTAGAAAGGAAAATCATCT | PstI | Amplification of ′gusA |
| gusARev | AAAAACCTAGGTATTAATTTAATTGTTGCCA | XmaJI | Amplification of ′gusA |
| IVET-EclF | TGACTGAGCTCGAGCTTCTGTTTTGGCG | SacI | Amplification of IVETgus cassette |
| IVET-EclR | TGACTGAGCTCCAAGATTGTTTAAGCAAAATAAG | SacI | Amplification of IVETgus cassette |
| ldhL1 | AGTCGACGTTTGTTATTGC | Amplification of ldhL promoter | |
| ldhL2 | CAATTCTTCATTTCGAAAAC | Amplification of ldhL promoter | |
| IVETFor | CATTCAAATATGTATCCGCTC | Sequencing primer | |
| IVETgusRev | AGTGCCATTCATTAAAGTGT | Sequencing primer | |
| asnFor | GATCTGCAGTAACGAATGGCTTATGAAGGAT | PstI | Creation of L. sakei asnA2 mutant |
| asnRev | GATGTCGACTCATCAAATCTTCGCCTAAA | SalI | Creation of L. sakei asnA2 mutant |
| asn_controlFor | ACGCTTTTTTATTTTGGAAA | Control of L. sakei asnA2 mutant | |
| clpCFor | GATCTGCAGTAAGGAACGCACTACGTGAACT | PstI | Creation of L. sakei clpC mutant |
| clpCRev | GATGTCGACTCAAAGTCTTGTGCACCAAC | SalI | Creation of L. sakei clpC mutant |
| clpC_controlFor | ATTTACACCAAGTGCCAAAA | Control of L. sakei clpC mutant | |
| ctsRFor | GATCTGCAGTAAATTGAAATTCGACGCTCCG | PstI | Creation of L. sakei ctsR mutant |
| ctsRRev | GATGTCGACAATACCAAATTCCCCTCTTTTT | SalI | Creation of L. sakei ctsR mutant |
| ctsR_controlFor | TGCAAAGTCAGAATATCTCGG | Control of L. sakei ctsR mutant | |
| terCFor | GATCTGCAGTAAGTTATTCGTTCTTCTGAAGATT | PstI | Creation of L. sakei LSA1637 mutant |
| terCRev | GATGTCGACGCTAAGACGGAATCAATCGAG | SalI | Creation of L. sakei LSA1637 mutant |
| terC_controlFor | CCTTTCTTTGAATTAAATAACTGGC | Control of L. sakei LSA1637 mutant | |
| lactamFor | GATCTGCAGTAAGTTGTGGAAGTTAACGACGA | PstI | Creation of L. sakei LSA1065 mutant |
| lactamRev | GATGTCGACGCCTGCGCGATAAATCAT | SalI | Creation of L. sakei LSA1065 mutant |
| lactam_controlFor | ATGCCACTTGGTGGTGTACGC | Control of L. sakei LSA1065 mutant | |
| erfKFor | GATCTGCAGTAAAAAATGAAAAAAAGCCCTTCA | PstI | Creation of L. sakei LSA1649 mutant |
| erfKRev | GATGTCGACTTAAAGAAGGCTTCACCGTG | SalI | Creation of L. sakei LSA1649 mutant |
| erfK_controlFor | GCGATTACCGCAAGCTATCC | Control of L. sakei LSA1649 mutant | |
| nodFor | GATCTGCAGTAAATTAACGTCATGCGAGCCA | PstI | Creation of L. sakei LSA1194 mutant |
| nodRev | GATGTCGACCATAAGGATTTGTGAAGGCGT | SalI | Creation of L. sakei LSA1194 mutant |
| nod_controlFor | TCGGTTGAAGTATTACAAATCGT | Control of L. sakei LSA1194 mutant | |
| DH05 | ACGACGTTGTAAAACGACGGCCAG | Control of L. sakei mutants |
a
Recognition sites for restriction endonucleases are underlined.