TABLE 2.
Day | Tetrads (T15 = 3.6, T100 = 18.7)
|
Vegetative cells (T15 = 2.25, T100 = 16)
|
||||||
---|---|---|---|---|---|---|---|---|
CFU/ml | % with phenotype:
|
CFU/ml | % with phenotype:
|
|||||
G418R | SMRR | G418R + SMRR | G418R | SMRR | G418R + SMRR | |||
2 | 3.5 × 105 ± 1 × 103 | 15 ± 0.6 | 13 ± 1.1 | 72 ± 0.6 | 3 × 106 ± 2 × 105 | 0 ± 0 | 0 ± 0 | 100 ± 0 |
4 | 2.1 × 107 ± 2 × 106 | 15 ± 1.7 | 30 ± 0.5 | 55 ± 1.1 | 1 × 107 ± 8 × 105 | 0 ± 0 | 0 ± 0 | 100 ± 0 |
8 | 1.9 × 107 ± 9 × 105 | 13 ± 0.4 | 35 ± 1.1 | 52 ± 1 | 1.5 × 107 ± 2 × 106 | 0 ± 0 | 0 ± 0 | 100 ± 0 |
11 | 2.8 × 107 ± 1 × 106 | 26 ± 0.4 | 23 ± 0.4 | 51 ± 0.7 | 2.2 × 107 ± 6 × 105 | 0 ± 0 | 0 ± 0 | 100 ± 0 |
G418R, resistant to G418; SMRR, resistant to SMR; G418R + SMRR, resistant to both G418 and SMR. The data are the mean values (percentages) of two independent experiments and standard errors. One hundred to 300 colonies were analyzed from each sample. The first sample was taken on the second day of fermentation because of the time needed for spore germination, mating, and yeast population growth.