TABLE 3.

Analysis of the frequency of genetic markers during the second fermentation of must inoculated with tetrads (from a sporulated culture of the yeasts from day 2 of the first fermentation) and vegetative cells of the H77 hybrid^a

Day	Tetrads (T15 = 3, T100 = 15)				Vegetative cells (T15 = 1.5, T100 = 11)
	CFU/ml	% with phenotype:			CFU/ml	% with phenotype:
		G418R	SMRR	G418R + SMRR		G418R	SMRR	G418R + SMRR
2	4.3 × 105 ± 2 × 103	20 ± 0.5	26 ± 0.9	54 ± 0.5	1.3 × 105 ± 8 × 103	0 ± 0	0 ± 0	100 ± 0
7	6.2 × 107 ± 2 × 105	13 ± 0.9	18 ± 0.6	69 ± 1.4	2 × 106 ± 1 × 105	0 ± 0	0 ± 0	100 ± 0
11	6.2 × 107 ± 8 × 105	19 ± 0.9	17 ± 0.1	64 ± 0.8	7.4 × 107 ± 7 × 105	0 ± 0	0 ± 0	100 ± 0
21	6.3 × 107 ± 1 × 105	21 ± 0.7	7.4 ± 1.3	71 ± 1	9.4 × 107 ± 2 × 105	0 ± 0	0 ± 0	100 ± 0

^aG418^R, resistant to G418; SMR^R, resistant to SMR; G418^R + SMR^R, resistant to both G418 and SMR. The data are the mean values (percentages) of two independent experiments and standard errors. One hundred to 300 colonies were analyzed from each sample. The first sample was taken on the second day of fermentation because of the time needed for spore germination, mating, and yeast population growth.