TABLE 2.
Growth rates, final OD600 values, and total folate production levels of the wild-type and NZ9000ΔpABA strains grown on CDM with and without pABA and nucleobases/nucleosides
| Presence of pABA | Presence of nucleobase/nucleoside | Wild type
|
NZ9000ΔpABA
|
Mean concn (μg/liter) of folate (SD) produced in:
|
|||
|---|---|---|---|---|---|---|---|
| Mean growth rate (h−1) (SD) | OD600 | Mean growth rate (h−1) (SD) | OD600 | Wild type | NZ9000ΔpABA | ||
| − | − | 0.31 (0.03) | 3.2 | 0.02 (0.001) | 0.5 | 91.7 (10.5) | 0.05 (0.01)a |
| + | − | 0.31 (0.01) | 3.3 | 0.29 (0.03) | 3.1 | 129.9 (7.9) | 92.0 (12.3) |
| − | + | 0.32 (0.05) | 3.5 | 0.26 (0.04) | 3.3 | 103.7 (9.6) | 0.05 (0.01)a |
| + | + | 0.32 (0.03) | 3.5 | 0.35 (0.06) | 3.3 | 90.3 (16.0) | 109.7 (10.3) |
a
This sample was concentrated 500-fold prior to its addition in the microbiological assay. The actual concentration given in this table is the calculated value based on analysis of the concentrated sample in the assay.