TABLE 1.
Putative human-specific PCR assay primers, optimal annealing temperatures, and target DNA annotation
| PCR assay no. | Primer set | Sequences (5′ → 3′) | Amplicon length (bp) | Annealing temp (°C) | Target DNA annotation |
|---|---|---|---|---|---|
| 1 | hum5Fc | CCGGCGGTGGCTTTGACTA | 116 | 56 | Putative tricorn-like protease |
| hum5Rc | TCCTCCTTGTGCACTTACCATACC | ||||
| 2 | hum12Fb | GCAGGAAGGCAAATGGTT | 133 | 63 | Periplasmic beta-glucosidase precursor |
| hum12Rb | AGCAGATGCACTCAGGGCGATGCTC | ||||
| 3 | hum13Fa | TCATCATCCTCAAGGCGAACAAT | 115 | 58 | Serine protease precursor |
| hum13Ra | GGGAACATACCGGTGATAAACAAC | ||||
| 4 | hum14Fb | CATCTCCGGACTTGCCATTACTT | 127 | 57 | Putative membrane protein |
| hum14Rb | TTCCGCTCCTTTTATATCTTTCTG | ||||
| 5 | hum24Fb | CATCGGTGGTTCCCTTCAGTC | 141 | 61 | Putative outer membrane protein |
| hum24Rb | TCTTGGGATGGGTTTTTGGTAGTA | ||||
| 6 | hum33Fb | CGGATGTATCGGCAGGTA | 121 | 59 | Putative outer membrane protein |
| hum33Rb | CCGTTTCATAGTTCCAAGCATTAG | ||||
| 7 | hum39Fc | GCCATGAGAAGTTTGCAGAGATAG | 100 | 64 | Putative outer membrane protein |
| hum39Rc | TTGGGAGAAATGGAAAATACC | ||||
| 8 | hum63Fc | CATGAGGAATACTGCCCACTGAAT | 206 | 62 | Putative exported hydrolase |
| hum63Rc | TCCCAATGAACCACGAGACG | ||||
| 9 | hum64Fa | ACCGGTACCTGTTCGTTTGTGT | 228 | 61 | Xanthan lyase |
| hum64Ra | CATTGGCGGTGAAGTTTGTATCT | ||||
| 10 | hum70Fb | CATCACCGTGCAGCAGTATTAGG | 189 | 59 | Putative oligopeptide transporter |
| hum70Rb | AAGTTCGGGTGACATTTCGCTGAT | ||||
| 13 | hum86Fb | TAATGGAAGGATAGAATAAATAGT | 99 | 55 | Putative outer membrane protein |
| hum86Rb | AAAGGACAAAGCCAAAGCATA | ||||
| 14 | hum135Fc | TGGGCATTTACTTCATCC | 101 | 56 | Putative outer membrane protein |
| hum135Rc | GAGCATTTCCCGACAGA | ||||
| 15 | hum137Fa | ACCGGTCCGCTTTATGTGATT | 163 | 58 | Putative outer membrane protein |
| hum137Ra | AACGACCGCCTTTAGTAGTGACC | ||||
| 16 | hum144Fa | TCTCTGCATGGCTGACA | 158 | 64 | Putative membrane efflux protein |
| hum144Ra | CGCTTTGGCTATATTGGGAGGTA | ||||
| 17 | hum153Fa | TGCGTGGTACTAAATCTATCAT | 97 | 60 | Putative outer membrane protein |
| hum153Ra | AACTCTGTACCTCCTTCATTTGT | ||||
| 18 | hum162Fb | CAACGTAAACTCTCGGGTGATAA | 106 | 60 | Putative TonB-dependent receptor |
| hum162Rb | CGGTGCCAGCGGTAAGTTT | ||||
| 19 | hum163Fa | CGTCAGGTTTGTTTCGGTATTG | 165 | 60 | Hypothetical protein BF3236 |
| hum163Ra | AAGGTGAAGGTCTGGCTGATGTAA | ||||
| 20 | hum184Fa | TTGCCGCCAGATTCATAAAAA | 130 | 65 | Putative transmembrane spore maturation- |
| hum184Ra | AAGATAGGCGAGAAAGGGGGAGTC | like protein | |||
| 21 | hum172Fa | GTTACGGTACGCAGAAGAAGGTGA | 142 | 62 | Putative outer membrane protein |
| hum172Ra | CCCGACGAGGTAGTGACATT | ||||
| 22 | hum181Fb | GTAATTCGCGTTCTTCCTCACAT | 110 | 61 | Putative RNA polymerase extracytoplasmic function- |
| hum181Rb | ACCTGCAAACCGTACAAGAAAAA | type sigma factor | |||
| 24 | hum218Fb | TCAATTTTACCACGCCAGAA | 184 | 55 | Putative outer membrane protein |
| hum218Rb | CTACGCAAGATGAATATGAAGGTG | ||||
| 26 | hum243Fb | TTCCGGCATCTGTTCTACTATCTC | 97 | 64 | TonB |
| hum243Rb | CAATCAGGCTGTGGAAATCAAA | ||||
| 27 | hum245Fc | GCGGATGTCGAGCAGGAAAGTC | 98 | 60 | Glycosyltransferase |
| hum245Rc | GCTACCGGGGAGAAACCAAAGAAC | ||||
| 28 | hum327Fa | CGCATGGGCCGGATTTACG | 124 | 62 | Polysaccharide biosynthesis protein |
| hum327Ra | CACCGCAGCCAACAGCACATAG | ||||
| 29 | hum330Fb | CATCGCCCTTATCTTGGTT | 94 | 63 | Putative biopolymer transport protein |
| hum330Rb | TGGCGTATTAGCAGGTTCA | ||||
| 30 | hum336Fa | CCAACGGCGTAACTTCTTCA | 162 | 62 | Outer membrane efflux protein precursor |
| hum336Ra | ATTACCGGATTACAAACCTTATG |