TABLE 1.
Pseudomonas strains used in this study and identification by species-specific carA PCR assay
| Species | Strain | Sourcea | Origin | Reference |
carA PCR result for:
|
||
|---|---|---|---|---|---|---|---|
| P. putida | P. fragi | P. lundensis | |||||
| P. fragi | DSM3456T | DSMZ | − | + | − | ||
| P. lundensis | DSM6252T | DSMZ | − | − | + | ||
| P. putida | DSM291T | DSMZ | + | − | − | ||
| P. aeruginosa | DSM50071T | DSMZ | − | − | − | ||
| P. aeruginosa | DSM27853 | DSMZ | − | − | − | ||
| P. agarici | DSM11810T | DSMZ | − | − | − | ||
| P. chlororaphis | DSM50082 | DSMZ | − | − | − | ||
| P. cichorii | DSM50259T | DSMZ | − | − | − | ||
| P. costantinii | DSM16734T | DSMZ | − | − | − | ||
| P. flavescens | DSM12071T | DSMZ | − | − | − | ||
| P. fluorescens | DSM50091 | DSMZ | − | − | − | ||
| P. fluorescens | DSM50108 | DSMZ | − | − | − | ||
| P. fluorescens | DSM50415 | DSMZ | − | − | − | ||
| P. marginalis | DSM13124T | DSMZ | − | − | − | ||
| P. putida biotype A | DSM50208 | DSMZ | + | − | − | ||
| P. putida biotype B | DSM50222 | DSMZ | −c | − | − | ||
| P. stutzeri | DSM5190T | DSMZ | − | − | − | ||
| P. syringae | DSM1241 | DSMZ | − | − | − | ||
| P. syringae | DSM10604 | DSMZ | − | − | − | ||
| P. fragi | PMK37 | DTA | Cheese | 16 | − | + | − |
| P. lundensis | PMK52 | DTA | Cheese | 16 | − | − | + |
| P. putida | PMK32 | DTA | Cheese | 16 | − | − | − |
| Pseudomonas sp. | L1114 | DFST | Fish | 19 | − | + | − |
| Pseudomonas sp. | L414 | DFST | Fish | 19 | − | + | − |
| Pseudomonas sp. | L47 | DFST | Fish | 19 | − | + | − |
| Pseudomonas sp. | L514 | DFST | Fish | 19 | − | − | − |
| Pseudomonas sp. | L128 | DFST | Fish | 19 | − | − | − |
| Pseudomonas sp. | L1110 | DFST | Fish | 19 | − | − | − |
| Pseudomonas sp. | 25P | DSAb | Meat | − | − | − | |
| P. fragi | 24P | DSA | Meat | − | + | − | |
| P. fragi | 26P | DSA | Meat | − | + | − | |
| P. fragi | 27P | DSA | Meat | − | + | − | |
| Pseudomonas sp. | 28P | DSA | Meat | − | − | − | |
| Pseudomonas sp. | 29P | DSA | Meat | − | − | − | |
| P. fragi | 30P | DSA | Meat | − | + | − | |
| Pseudomonas sp. | 33M | DSA | Meat | − | − | − | |
| Pseudomonas sp. | 39M | DSA | Meat | − | − | − | |
| Pseudomonas sp. | 1P2 | DSA | Cheese | − | − | − | |
| Pseudomonas sp. | 6P2 | DSA | Cheese | − | − | + | |
| P. fluorescens | PSEflu4 | DISTAM | Milk | − | − | − | |
| P. fluorescens | PSEflu24 | DISTAM | Salad | − | − | − | |
| P. fluorescens | PSEflu13 | DISTAM | Salad | − | − | − | |
| P. fluorescens biovar C | PSEflu14 | DISTAM | Salad | − | − | − | |
| P. fluorescens biovar G | PSEflu20 | DISTAM | Salad | − | − | − | |
| P. fluorescens | PSEflu22 | DISTAM | Salad | − | − | − | |
| P. fragi | 1S 63 | VSA | Fish | 8 | − | + | − |
| P. fragi | 4S 72 | VSA | Fish | 8 | − | + | − |
| P. fragi | F188 | VSA | Meat | 9 | − | + | − |
| P. fragi | F271 | VSA | Fish | 9 | − | + | − |
| P. fragi | 101M5 | VSA | Meat | 9 | − | + | − |
| P. lundensis | F31 | VSA | Meat | 9 | − | − | + |
| P. putida biotype A | F385 | VSA | 9 | − | − | − | |
| P. putida biotype A | F164 | VSA | 9 | + | − | − | |
| P. putida biotype A | F196 | VSA | 9 | + | − | − | |
| P. putida biotype B | F292 | VSA | 9 | − | − | − | |
DSMZ, Deutsche Sammlung von Mikroorganismen und Zelkulturen, Braunschweig, Germany; DTA, Departamento de Tecnologia de Alimentos, INIA, Madrid, Spain; DFST, Department of Food Science and Technology, University of Athens, Athens, Greece; DSA, Dipartimento di Scienza degli Alimenti Università degli Studi di Napoli Federico II, Portici, Italy; DISTAM, Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università di Milano, Milano, Italy; VSA, Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università di Milano, Milano, Italy.
Strains previously identified by Microlog System 2 (Biolog, Hayward, CA) according to the manufacturer's instructions.
A spurious PCR product of less than 200 bp was detected after amplification of genomic DNA from this strain.