TABLE 2.
Effects of various factors on bacteriocin produced by W. cibaria 110
| Factor | OD660a of W. cibaria culture | Diam (mm) of zone of inhibitionb |
|---|---|---|
| Growth temp (°C) | ||
| 15 | 0.125 | − |
| 20 | 1.364 | − |
| 25 | 1.419 | 12 |
| 30 | 1.502 | 13 |
| 37 | 1.394 | 11 |
| 45 | 0.169 | − |
| Treatment with enzyme | ||
| Control | 13 | |
| Proteinase K | − | |
| Trypsin | − | |
| Catalase | 12 | |
| Heat | ||
| Control | 13 | |
| 30 min at 80°C | 13 | |
| 30 min at 90°C | 13 | |
| 30 min at 100°C | 13 | |
| 15 min at 110°C | 13 | |
| 15 min at 121°C | 13 |
a
OD660, optical density at 660 nm.
b
Wells (8 mm in diameter) were filled with 100 μl of supernatant from the W. cibaria 110 culture. L. sakei JCM 1157T was used as the indicator strain. −, no inhibitory zone observed.