TABLE 2.
Oligonucleotides used in this studya
| Primer | DNA sequence (5′ to 3′) | Nucleotide location of 5′ end |
|---|---|---|
| Q1 | CTGGAATTCTAGCATCGAGGGCAGGACATCAGA | −1450 |
| Q5 | CTGGAATTCATATGAGCTTTCTCGTGTCCGAGG | +1 |
| Q6 | CTGAAGCTTCACCGCTGCCTTGCTGGCCGGGTC | +540 |
| ssgA W41A | CCGAAGGCCGCGGTCACCGGCGCGTC | +105 |
| ssgA S89A | CGCGGCGGAGGCACGGAACAGCG | +254 |
| ssgA L94A | GAAGGCCACCGCGGGCGCGGCG | +270 |
| ssgA P106A | CCTGCCCCAGCGCCACCAGCTTG | +308 |
a
Restriction sites used for cloning are presented in bold. Restriction sites: GAATTC, EcoRI; AAGCTT, HindIII; CATATG, NdeI. Underlined triplets are present to introduce codons encoding alanine. The locations of the 5′ ends of oligonucleotides are given relative to the start of ssgA.