TABLE 3.
Single-amino-acid mutations in a collection of clones obtained for the library expressing mutant SsgAa
| Mutation | Sporulation | Mutation | Sporulation | |
|---|---|---|---|---|
| Y25C | Not restored | Q79R | Restored | |
| V27L | Not restored | S82C | Restored | |
| R28C | Restored | A85P | Not restored | |
| L29P | Not restored | L86P | Not restored | |
| L29Q | Not restored | F87S | Not restored | |
| T30A | Restored | S89A* | Restored | |
| T30S | Restored | S90P | Restored | |
| F31L | Restored | A92V | Restored | |
| F31S | Restored | L94A* | Not restored | |
| L33P | Restored | V95A | Restored | |
| D36G | Restored | L98H | Not restored | |
| D36N | Restored | D99E | Restored | |
| D36V | Restored | D99G | Restored | |
| P38L | Restored | T101A | Restored | |
| T40A | Restored | D102N | Restored | |
| W41A* | Not restored | K103E | Restored | |
| G44D | Restored | K103M | Restored | |
| R45L | Not restored | L104P | Not restored | |
| E46G | Restored | L104V | Restored | |
| L47Q | Not restored | V105M | Restored | |
| L48P | Not restored | P106A* | Restored | |
| D50V | Restored | L107P | Restored | |
| G51R | Not restored | G111D | Restored | |
| V52A | Restored | G111S | Restored | |
| G53S | Restored | L113F | Restored | |
| R54Q | Restored | L113P | Restored | |
| C56R | Restored | A114T | Restored | |
| G57S | Not restored | F116L | Restored | |
| R62G | Restored | F116S | Restored | |
| I63T | Not restored | S118G | Restored | |
| P65R | Restored | H119P | Restored | |
| P70Q | Not restored | D121V | Restored | |
| P70L | Restored | L124P | Not restored | |
| L71P | Restored | D125V | Restored | |
| V74A | Not restored | I127T | Restored | |
| V74E | Not restored | L128P | Restored | |
| L75P | Not restored | S133C | Restored | |
| I76T | Not restored | A134E | Restored | |
| R77G | Restored | G135D | Restored | |
| R77Q | Restored | G135S | Restored | |
| L78P | Not restored |
a
Asterisks indicate point mutations made by site-directed mutagenesis.