TABLE 3.

Single-amino-acid mutations in a collection of clones obtained for the library expressing mutant SsgA^a

Mutation	Sporulation		Mutation	Sporulation
Y25C	Not restored		Q79R	Restored
V27L	Not restored		S82C	Restored
R28C	Restored		A85P	Not restored
L29P	Not restored		L86P	Not restored
L29Q	Not restored		F87S	Not restored
T30A	Restored		S89A*	Restored
T30S	Restored		S90P	Restored
F31L	Restored		A92V	Restored
F31S	Restored		L94A*	Not restored
L33P	Restored		V95A	Restored
D36G	Restored		L98H	Not restored
D36N	Restored		D99E	Restored
D36V	Restored		D99G	Restored
P38L	Restored		T101A	Restored
T40A	Restored		D102N	Restored
W41A*	Not restored		K103E	Restored
G44D	Restored		K103M	Restored
R45L	Not restored		L104P	Not restored
E46G	Restored		L104V	Restored
L47Q	Not restored		V105M	Restored
L48P	Not restored		P106A*	Restored
D50V	Restored		L107P	Restored
G51R	Not restored		G111D	Restored
V52A	Restored		G111S	Restored
G53S	Restored		L113F	Restored
R54Q	Restored		L113P	Restored
C56R	Restored		A114T	Restored
G57S	Not restored		F116L	Restored
R62G	Restored		F116S	Restored
I63T	Not restored		S118G	Restored
P65R	Restored		H119P	Restored
P70Q	Not restored		D121V	Restored
P70L	Restored		L124P	Not restored
L71P	Restored		D125V	Restored
V74A	Not restored		I127T	Restored
V74E	Not restored		L128P	Restored
L75P	Not restored		S133C	Restored
I76T	Not restored		A134E	Restored
R77G	Restored		G135D	Restored
R77Q	Restored		G135S	Restored
L78P	Not restored

^aAsterisks indicate point mutations made by site-directed mutagenesis.