TABLE 2.
Bacterial ribosomal 16S rRNA clone library results for Osedax spp.a
| Microbial group and subgroup | No. of clones
|
Representative ribotype ID (GenBank accession no.) | ||
|---|---|---|---|---|
| Osedax frankpressib (2,895 m) | Osedax sp. MB3 (1,017 m) | Osedax sp. MB4 (381 m) | ||
| Oceanospirillales | 42 | 95 | 85 | |
| Phylotype 1 | 95 | T776.29.G3 (DQ911537) | ||
| T931.1.B1 (DQ911539) | ||||
| Phylotype 4 | 42 | T769r.1.B10 (DQ911535) | ||
| Phylotypes 5 and 6 | 85 | T933.1.G2 (DQ911544) | ||
| T933.2.H5 (DQ911547) | ||||
| Epsilonproteobacteria | 4 | 111 | 1 | |
| Arcobacter-related | 3 | 53 | T776.29.15 (EF100882) | |
| T776.29.C8 (EF100883) | ||||
| T931.1.A8 (EF100886) | ||||
| Sulfurimonas-related | 43 | T776.29.D3 (EF100884) | ||
| T776.29.H8 (EF100885) | ||||
| T931.1.H4 (EF100889) | ||||
| Sulfurospirillum-related | 15 | 1 | T776.29.11 (EF100881) | |
| T931.1.D7 (EF100888) | ||||
| Other groups | 4 | 53 | 5 | |
| CFB | 3 | 28 | 2 | T933.1.E3 (EF117251) |
| Low-G+C gram positive | 0 | 8 | 1 | T776.30.A8 (EF117250) |
| Gammaproteobacteria | 0 | 5 | 1 | T776.28.F8 (EF117249) |
| Unknown/other | 1 | 12 | 1 | NA |
| Total clones sequenced | 50 | 259 | 91 | |
a
Only dominant representative ribotypes are listed. Total numbers for each are shown in boldface type. There were three individual O. frankpressi juveniles, six individuals for Osedax sp. MB3, and two individuals for Osedax sp. MB4. Osedax sp. MB3 individuals were collected in January 2005 (dive T776) and January 2006 (dive T931). Phylotypes (P1 to P6) are internal clades within the Oceanospirillales-related symbionts of Osedax (Fig. 2). Ribotype names (dive.individual.clone) correspond to taxon labels in Fig. 2 and 3. NA, not applicable.
b
Osedax frankpressi juveniles from dive T769.